The fresh sanctions imposed on Iran target 13 individuals and 12 companies, including groups in China, Lebanon and the United Arab Emirates working to help Tehran obtain technology and materials required to advance its ballistic missile program.
Washington added more Iranian individuals and entities to its anti-Iran sanction list on Friday.
National Security Advisor Mike Flynn said Wednesday that Iran is "on notice" after ballistic missile tests and an attack by Iran-backed Houthi rebels on a Saudi vessel, though the White House did not detail what that notice meant.
These actions "underscore what should have been clear to the global community all along about Iran's destabilizing behavior across the Middle East", Michael Flynn, national security advisor to President Trump, said in a statement.
"We will continue to actively apply all available tools, including financial sanctions, to address this behavior", he said.
Gen. Amir Ali Haijazadeh said: "If the enemy does not walk the line, our missiles come down on them".
European Union leaders launch scathing attack on Donald Trump
Ms Merkel said she was "gratified" that the Prime Minister had said she wanted to see a "strong EU", even after Britain had left. But she added that there was also a "clear recognition" that Europe needs to step up its independent defense capability.
President Trump and his top aides have said they won't tolerate provocative acts like the missile test that occurred Sunday, calling it a violation of a United Nations Security Council resolution.
It said the names of the banned USA people and entities would be announced later. That's pretty much all we can say at this point.
Trump tweeted that Iran "is playing with fire" and " they don't appreciate how "kind" President Obama was to them.
Iranian English language Press TV reported that Iran Senior Vice President Ishaq Jahangiri dismissed what he called recent anti-Iran posturing by the U.S. He said "threadbare" accusations are aimed by Washington at scaring away investors.
Iran has a formidable arsenal of thousands of short-range and medium-range ballistic missiles capable of reaching Israel and other USA allies in the Middle East, as well as American bases.
One reason Mattis was on the outs with the Obama administration is that he supported tougher responses to Iranian mischief in the region.
The UN Security Council held an emergency meeting on January 31 and referred the matter to its committee on Iran for an investigation.
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